| @ | Kerr-Cregger | 3:56.85 | Crawford, Gale, Hunter, Kalawan | St. Augustine's | 2018 |
| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | |
|---|---|---|---|---|---|---|---|---|
| 1 | Radford |
A | Gunnell | Burkley | Miller | Price | 3:58.00 | |
| 2 | Radford |
B | Foster | Washington | Lane | Rivers | 3:58.00 | |
| 3 | Carson-Newman |
A | Talbut-Smith | Hegarty | Nelms | Davis | 4:08.30 | South Atlantic Outdoor Track & Field Championship |
| 4 | Concord |
A | Strachan | Smith | Strachan | Mills | 4:10.00 | |
| 5 | Columbia (S.C.) |
A | Walker | Washington | Wright | Echeverria | 4:11.21 | Phoenix Invitational 2019 |
| 6 | Columbia (S.C.) |
B | Bell | White-Kennedy | Wall | Timmons | 4:11.21 | Phoenix Invitational 2019 |
| 7 | Carson-Newman |
B | Naude | Lowe | Da'lesio | Nelson | 4:15.22 | |
| 8 | Mary Baldwin University |
A | Blagmon | Holmes | Musgrove | Wilson | 4:18.37 | USA South Track and Field Conference Championships |
| 9 | Concord |
B | Thomas | Shrader | Thompson | Parsons | 4:20.00 | |
| 10 | Roanoke |
A | Alexander | Ball | Barthol | Henson | 4:25.00 | |
| 11 | Randolph |
A | Penn | Barrington | Davis | Sheets | 4:25.32 |