| # | Athlete | Yr. | Team | Seed | Note | |
|---|---|---|---|---|---|---|
| 1 | 633 | MENTINK, Justin | SO | Michigan T | 52.01 | |
| 2 | 610 | SHEPPARD, Nathaniel | FR | UMES | 52.23 | |
| 3 | 1214 | PETRECCA, Jack | JR | Wooster | 52.34 | |
| 4 | 161 | WILLIAMS, Jabari | SO | Bethany | 52.51 | |
| 5 | 683 | SCHMELYUN, Dhane | FR | Mount St M | 52.52 | |
| 6 | 154 | KLEIN, Hunter | SO | Bethany | 52.93 | |
| 7 | 460 | BURELISON, Mitch | JR | Indiana E | 53.88 | |
| 8 | 1157 | SEWARD, Jonathan | FR | UNCW | 53.91 | |
| 9 | 844 | HAYWOOD, Hassan | JR | Rutgers | 54.15 | |
| 10 | 1001 | RAAB, Judah | FR | Swarthmore | 55.21 | |
| 11 | 338 | MUSE, Ryan | FR | Concord | 55.34 | |
| 12 | 155 | LUJAN, Jose | FR | Bethany | 55.63 | |
| 13 | 1216 | POLLARD, Camryn | FR | Wooster | 55.81 | |
| 14 | 842 | DIEUJUSTE, Theodore | FR | Rutgers | 56.23 | |
| 15 | 991 | BELGAUMI, Adil | SO | Swarthmore | 57.72 | |
| 16 | 574 | FRAZIER, Marcus | SO | Mansfield | 58.49 | |
| 17 | 851 | RIVERA, Daniel | FR | Rutgers | 59.06 | |
| 18 | 1209 | HELSEL, Thomas | SR | Wooster | 59.51 | |
| 19 | 839 | COLE, Nasir | FR | Rutgers | 1:03.24 | |
| 20 | 1186 | MCLELLAN, JD | U of Toronto | NT | ||
| 21 | 1188 | SHIVERS, Grant | U of Toronto | NT | ||
| 22 | 1184 | JOHNSON, Keith | U of Toronto | NT | ||
| 23 | 728 | KVIDERA, Scott | SR | N Dakota | NT | |
| 24 | 735 | RUSSELL, Matt | FR | N Dakota | NT | |
| 25 | 773 | MAZAKO, Nolan | FR | Post | NT |