| @ | Kerr-Cregger | 3:55.85 | Crawford, Gale, Hunter, Kalawan | St. Augustine's | 2018 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Roanoke |
A | Gheen | Koepfinger | Henson | Mallette | 4:26.71 | Roanoke Invitational Challenge |
| 4 | Southern Virginia |
A | Parker | Godfrey | Barton | Vanek | 4:25.93 | NYC DIII Invitational |
| 5 | Piedmont |
B | Mitchell | Lawrence | Granger | Carvallo | DNS | Buccaneer Track & Field Invitational 2019 |
| 6 | Carnegie Mellon |
B | Andrade | Sidwell | Tummalapalli | Yuan | 3:58.59 | UAA Outdoor Championship |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Piedmont |
A | Graham | Bradach | Bryan | Thigpen | 4:16.27 | Buccaneer Track & Field Invitational 2019 |
| 4 | Washington and Lee |
A | Teagle | Jones | Rocha | Cunningham | 4:09.84 | ODAC Outdoor Track & Field Championships |
| 5 | Carnegie Mellon |
A | Baranowski | Jacobs | Fahrenkrug | Giordani | 3:58.59 | UAA Outdoor Championship |
| 6 | Gettysburg |
A | Hill | Desmond | Cantrell | Larzelere | 4:22.29 | Centennial Conference Outdoor Championships |