| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Washington and Lee | B | Huffman, Carter | Jones, John | Agrippina, Andrew | Del Cristo, Albert | 3:33.00 | |
| 4 | Washington and Lee | A | Fiore, Donovan | Pranka, Drew | Roberts, Jake | Schlott, Max | 3:20.53 | High Point VertKlasse Meeting |
| 5 | Lynchburg | A | Cahill, Connor | Dorner-Thomas, Justin | Sharkey, Reid | Williams, Luke | DNS | |
| 6 | Lynchburg | B | Sprecher, Trace | Fleck, Jacob | Rees, Levi | Moran, Kevin | DNS |